Nikon Instruments Inc. has announced the winners of the forty-fifth annual Nikon Small World Photomicrography Competition. Below are the top 20 photos from more than 2,000 images submitted by scientists in 100 countries.
First place was awarded to microscopy technician Teresa Zgoda and recent university graduate Teresa Kugler for their visually stunning and painstakingly prepared photo of a turtle embryo. Captured using fluorescence and stereo microscopy, the colourful final image is a masterful example of image-stitching.
Image-stitching is an imaging technique that required the 2019 winning pair to stack and stitch together hundreds of images to create the final image of their turtle. Adding to the challenge was the size and thickness of the turtle embryo. Creating the final image required precision, patience, and deep imaging expertise, as the organism’s size meant only very small parts of the turtle could be imaged on the focal plane at a time.
Second place was awarded to Nikon Small World veteran Dr. Igor Siwanowicz for his composite image of three single-cell freshwater protozoans, sometimes called “trumpet animalcules.” He used confocal microscopy to capture the detail of the cilia, tiny hairs used by the animals for feeding and locomotion.
In third place is Mr. Daniel Smith Paredes, who placed for his image of a developing American alligator embryo. He snapped this photo at around 20 days of development using immunofluorescence and is studying the development and evolution of vertebrate anatomy.
‘The Nikon Small World competition has been bringing stunning scientific images to the public for 45 years now,’ said Eric Flem, Communications Manager, Nikon Instruments, ‘Our goal has always been to show the world how art and science intersect. As new imaging and microscopy techniques develop over the years, our winners showcase these technology advances more and more creatively. First place this year is no exception.’